Evaluation of three molecular carbapenemase tests: Eazyplex SuperBug complete B, Novodiag CarbaR+, and Amplidiag CarbaR+MCR
نویسندگان
چکیده
Carbapenemase-producing Gram-negative bacilli, i.e., Enterobacterales, Pseudomonas aeruginosa , and Acinetobacter are of increased concern for the public health around world. There is urgent need rapid accurate tests in order to provide correct treatment prevent bacterial spread healthcare settings. The aim this study was evaluate three commercial multiplex carbapenemase with CE-IVD marking: Eazyplex SuperBug complete B (AmplexDiagnostics), Novodiag CarbaR+ (Mobidiag), Amplidiag CarbaR+MCR (Mobidiag). All these recognize KPC, NDM, OXA-48/181 group, VIM, OXA-23 OXA-24/40 additionally IMP, OXA-51 group (with promoter located within ISAbaI), OXA-58 MCR, further recognizes GES (carbapenemase-type only). sensitivities specificities isolates were 100%. sensitivity directly from clinical samples 100%, but specificity lower, which simply explained by higher molecular methods compared culture method. Overall, marked a good alternative detection carbapenemase-producing organisms. • Detection carbapenemases essential prevention resistant bacteria. Novel regulation requires implementation tests. Eazyplex, Ampldiag reliable
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چکیده ندارد.
15 صفحه اولEvaluation of the eazyplex® SuperBug CRE system for rapid detection of carbapenemases and ESBLs in clinical Enterobacteriaceae isolates recovered at two Spanish hospitals.
OBJECTIVES To evaluate the performance of the eazyplex(®) SuperBug CRE system, a loop-mediated isothermal amplification (LAMP)-based system, for confirming the presence of carbapenemases in addition to CTX-M-type ESBLs in previously genotypically and/or phenotypically characterized clinical Enterobacteriaceae isolates recovered in two centres in Spain. METHODS A collection of 94 carbapenemase...
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OBJECTIVES To assess the performance of three commercial molecular assays for detecting major families of carbapenemases in pure bacterial isolates. METHODS A panel of 450 isolates with previously defined carbapenem resistance mechanisms was tested using the Check-Direct CPE kit, the eazyplex(®) SuperBug complete A kit and the Xpert(®) Carba-R kit. Isolates included 438 Enterobacteriaceae and...
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ژورنال
عنوان ژورنال: Journal of Microbiological Methods
سال: 2021
ISSN: ['0167-7012', '1872-8359']
DOI: https://doi.org/10.1016/j.mimet.2020.106105